Purification and Characterization of Phosphoribulokinase from the Marine Chromophytic Alga Heterosigma carterae1
نویسندگان
چکیده
منابع مشابه
Purification and characterization of phosphoribulokinase from the marine chromophytic alga Heterosigma carterae.
In this study we characterized phosphoribulokinase (PRK, EC 2.7.1. 19) from the eukaryotic marine chromophyte Heterosigma carterae. Serial column chromatography resulted in approximately 300-fold purification of the enzyme. A polypeptide of 53 kD was identified as PRK by sequencing the amino terminus of the protein. This protein represents one of the largest composite monomers identified to dat...
متن کاملProtein R-phycoerythrin from marine red alga Amphiroa anceps: extraction, purification and characterization
The paper reports an efficient procedure for the extraction and purification of R-phycoerythrin (R-PE) from marine red algae Amphiroa anceps. A. anceps was extracted in 50 mM phosphate buffered saline and precipitated by (NH4)2SO4. The R-PE was isolated by ion-exchange chromatography loaded with Q-Sepharose Fast Flow, which was developed by linear ionic strength gradients. Then it was purified ...
متن کاملthe role of russia in transmission of energy from central asia and caucuses to european union
پس ازفروپاشی شوروی،رشد منابع نفت و گاز، آسیای میانه و قفقاز را در یک بازی ژئوپلتیکی انرژی قرار داده است. با در نظر گرفتن این منابع هیدروکربنی، این منطقه به یک میدانجنگ و رقابت تجاری برای بازی های ژئوپلتیکی قدرت های بزرگ جهانی تبدیل شده است. روسیه منطقه را به عنوان حیات خلوت خود تلقی نموده و علاقمند به حفظ حضورش می باشد تا همانند گذشته گاز طبیعی را به وسیله خط لوله مرکزی دریافت و به عنوان یک واس...
15 صفحه اولStructural, Functional, and Evolutionary Analysis of Ribulose-1,5-Bisphosphate Carboxylase from the Chromophytic Alga Olisthodiscus luteus.
Ribulose-1,5-bisphosphate carboxylase (RuBPCase) was purified from the marine chromophyte Olisthodiscus luteus. This study represents the first extensive analysis of RuBPCase from a chromophytic plant species as well as from an organism where both subunits of the enzyme are encoded on the chloroplast genome. The size of the purified holoenzyme (17.9 Svedberg units, 588 kilodaltons) was determin...
متن کاملChlamydomonas reinhardtii Phosphoribulokinase : Sequence, Purification, and Kinetics.
The sequence and kinetic properties of phosphoribulokinase purified from Chlamydomonas reinhardtii were determined and compared with the spinach (Spinacea oleracea) enzyme. Chlamydomonas phosphoribulokinase was purified to apparent homogeneity, with a specific activity of 410 micromoles per minute per milligram. Polyclonal antibodies to the purified protein were used to isolate a Chlamydomonas ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1998
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.117.1.321